Often abbreviated to UV/Vis or UV-Vis.
UV/Visible spectroscopy offers the maximum flexibility and is suitable for
applications in the wavelength range 190 to 1100nm. In UV/Visible spectroscopy
the UV region is considered to be any wavelength less than 340nm. Nucleic acid,
purified proteins and other organic molecules are often measured in the UV
Often abbreviated to Vis. Visible
spectrophotometers and colorimeters typically measure in the visible region of
the electromagnetic spectrum (340-750nm). If your samples are colored this is
an indication that they will absorb visible light. If you do not need to
measure in the UV region (<340nm) a visible spectrophotometer or colorimeter
may be a more economical alternative.
MRC's spectrophotometers uv/vis, vis with many options like Kinetics , Scanning
, DNA protein Multi wavelengths etc.
There are two major classes of devices: single
beam and double
beam. A double beam spectrophotometer compares the light
intensity between two light paths, one path containing a reference sample and
the other the test sample. A single-beam spectrophotometer measures the
relative light intensity of the beam before and after a test sample is
inserted. Although comparison measurements from double-beam instruments are
easier and more stable, single-beam instruments can have a larger dynamic range
and are optically simpler and more compact.
Single Beam Spectrophotometer: